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Fig. 4 Significantly elevated PTEC <t>IL-1RI</t> expression and senescence in fibrotic kidneys. A Left panel: Quantitative analysis of % IL-1RI+ PTECs (proportion of AQP-1+ cells (i.e., PTECs) expressing IL-1RI) in control (non-fibrotic; n = 4) and fibrotic kidney tissue (n = 5). Points represent values for individual donor tissue samples. Results represent mean ± SEM of values from four randomly selected areas for each tissue sample. *P < 0.05, Welch’s t-test. Right panel: Representative immunofluorescent labelling of control (non-fibrotic) and fibrotic kidney tissue stained for PTEC marker aquaporin-1 (AQP-1; white), IL-1RI (green) and DAPI (blue). Scale bars represent 20 µm. IL-1RI+ PTECs are highlighted with white arrows. B Left panel: Quantitative analysis of % SA-β-gal+ PTECs (proportion of AQP-1+ cells (i.e., PTEC) with positive Spider-β-gal staining) in control (non-fibrotic; n = 4) and fibrotic kidney tissue (n = 4). Points represent values for individual donor tissue samples. Results represent mean ± SEM of values from four randomly selected areas for each tissue sample. **P < 0.01, Welch’s t-test. Right panel: Representative immunofluorescent images of control (non-fibrotic) and fibrotic kidney tissue labelled with PTEC marker AQP-1 (white), Spider-β-gal (green) and DAPI (blue). Scale bars represent 20 µm. SA-β-gal+ PTECs are highlighted with white arrows. Lower magnification immunofluorescent images are presented in Fig. S4.
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Fig. 4 Significantly elevated PTEC <t>IL-1RI</t> expression and senescence in fibrotic kidneys. A Left panel: Quantitative analysis of % IL-1RI+ PTECs (proportion of AQP-1+ cells (i.e., PTECs) expressing IL-1RI) in control (non-fibrotic; n = 4) and fibrotic kidney tissue (n = 5). Points represent values for individual donor tissue samples. Results represent mean ± SEM of values from four randomly selected areas for each tissue sample. *P < 0.05, Welch’s t-test. Right panel: Representative immunofluorescent labelling of control (non-fibrotic) and fibrotic kidney tissue stained for PTEC marker aquaporin-1 (AQP-1; white), IL-1RI (green) and DAPI (blue). Scale bars represent 20 µm. IL-1RI+ PTECs are highlighted with white arrows. B Left panel: Quantitative analysis of % SA-β-gal+ PTECs (proportion of AQP-1+ cells (i.e., PTEC) with positive Spider-β-gal staining) in control (non-fibrotic; n = 4) and fibrotic kidney tissue (n = 4). Points represent values for individual donor tissue samples. Results represent mean ± SEM of values from four randomly selected areas for each tissue sample. **P < 0.01, Welch’s t-test. Right panel: Representative immunofluorescent images of control (non-fibrotic) and fibrotic kidney tissue labelled with PTEC marker AQP-1 (white), Spider-β-gal (green) and DAPI (blue). Scale bars represent 20 µm. SA-β-gal+ PTECs are highlighted with white arrows. Lower magnification immunofluorescent images are presented in Fig. S4.
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Fig. 4 Significantly elevated PTEC IL-1RI expression and senescence in fibrotic kidneys. A Left panel: Quantitative analysis of % IL-1RI+ PTECs (proportion of AQP-1+ cells (i.e., PTECs) expressing IL-1RI) in control (non-fibrotic; n = 4) and fibrotic kidney tissue (n = 5). Points represent values for individual donor tissue samples. Results represent mean ± SEM of values from four randomly selected areas for each tissue sample. *P < 0.05, Welch’s t-test. Right panel: Representative immunofluorescent labelling of control (non-fibrotic) and fibrotic kidney tissue stained for PTEC marker aquaporin-1 (AQP-1; white), IL-1RI (green) and DAPI (blue). Scale bars represent 20 µm. IL-1RI+ PTECs are highlighted with white arrows. B Left panel: Quantitative analysis of % SA-β-gal+ PTECs (proportion of AQP-1+ cells (i.e., PTEC) with positive Spider-β-gal staining) in control (non-fibrotic; n = 4) and fibrotic kidney tissue (n = 4). Points represent values for individual donor tissue samples. Results represent mean ± SEM of values from four randomly selected areas for each tissue sample. **P < 0.01, Welch’s t-test. Right panel: Representative immunofluorescent images of control (non-fibrotic) and fibrotic kidney tissue labelled with PTEC marker AQP-1 (white), Spider-β-gal (green) and DAPI (blue). Scale bars represent 20 µm. SA-β-gal+ PTECs are highlighted with white arrows. Lower magnification immunofluorescent images are presented in Fig. S4.

Journal: Cell death & disease

Article Title: Human proximal tubular epithelial cell interleukin-1 receptor signalling triggers G2/M arrest and cellular senescence during hypoxic kidney injury.

doi: 10.1038/s41419-025-07386-6

Figure Lengend Snippet: Fig. 4 Significantly elevated PTEC IL-1RI expression and senescence in fibrotic kidneys. A Left panel: Quantitative analysis of % IL-1RI+ PTECs (proportion of AQP-1+ cells (i.e., PTECs) expressing IL-1RI) in control (non-fibrotic; n = 4) and fibrotic kidney tissue (n = 5). Points represent values for individual donor tissue samples. Results represent mean ± SEM of values from four randomly selected areas for each tissue sample. *P < 0.05, Welch’s t-test. Right panel: Representative immunofluorescent labelling of control (non-fibrotic) and fibrotic kidney tissue stained for PTEC marker aquaporin-1 (AQP-1; white), IL-1RI (green) and DAPI (blue). Scale bars represent 20 µm. IL-1RI+ PTECs are highlighted with white arrows. B Left panel: Quantitative analysis of % SA-β-gal+ PTECs (proportion of AQP-1+ cells (i.e., PTEC) with positive Spider-β-gal staining) in control (non-fibrotic; n = 4) and fibrotic kidney tissue (n = 4). Points represent values for individual donor tissue samples. Results represent mean ± SEM of values from four randomly selected areas for each tissue sample. **P < 0.01, Welch’s t-test. Right panel: Representative immunofluorescent images of control (non-fibrotic) and fibrotic kidney tissue labelled with PTEC marker AQP-1 (white), Spider-β-gal (green) and DAPI (blue). Scale bars represent 20 µm. SA-β-gal+ PTECs are highlighted with white arrows. Lower magnification immunofluorescent images are presented in Fig. S4.

Article Snippet: Sections were subsequently probed with primary antibodies against IL-1RI (1:70; Goat polyclonal IgG; Cat. No. AF269SP; R&D Systems, Minneapolis, MN, USA) and PTEC marker aquaporin-1 (AQP-1; 1:50; Mouse monoclonal IgG; Clone B-11; Cat. No. sc-25287; Santa Cruz, Dallas, TX, USA) overnight at 4 °C in a humidified chamber.

Techniques: Expressing, Control, Staining, Marker